Hippocampal slice culture protocol
Hippocampal slice culture protocol:
Materials and buffers to prepare:
1.Dissection solution: Gey's balanced sal solution supplemented with D-glucose to a concentration of 6.5mg/ml. (pH=7.1, 340m OSM)
Basal Medium Eagle Medium 50%
Earle's Balanced Salt Solution 25%
Horse serum 15%
(pH=7.1, 320m OSM)
Add before each use:
Glucose, to a volume ratio of 45%
PS(0.5ml/50ml for PS of 5000U/ml)
1. Kill the post-natal rat following the experimental animal rules.
2. Dissect the brains under dissection microscope and take the hippocampus out.
3. Cut the hippocampus into 40μm slices using microtome.